研究・業績

研究テーマ

　生物活性植物成分の単離・構造決定

地球上には多くの植物があり，漢方，中医学をはじめ多くの伝統医学では植物をくすりとして用いています。有効成分の研究は長い歴史がありますが，まだ手付かずの植物や，分析技術の進歩，新たな作用機構の解明などで改めて有用成分探索が必要な植物があります。我々hは生薬の生物活性成分の探索を進めているほか，インド，中国，トルコなどのアジアの研究者と連携して植物成分の探索を進めています。

　天然機能性食品に含まれる微量成分の同定

食品添加物やたびたびブームをおこす「健康食品」の中には含有成分が明らかとなっていないものも多くあります。食品の安全性確保の観点からこれらの含有成分を解明するとともに，有効性を持つ成分も含有されている可能性があることから有用物質の探索も同時に行なっています。

　生薬の品質評価に関する研究

生薬は天然の薬であるがゆえに品質をきちんと評価することが難しい薬でもあります。微量成分が必ずしもはっきりしていないものや，含有量の定量法がはっきりしていない成分もあります。これらの問題に関して，天然物化学の立場から取り組んでいます。

最近の学会発表

qHNMRによる生薬成分の定量

○田中理恵，羽佐田桂子，長谷部裕子，山崎麻里奈，仁田朱音，水上　元，永津明人

第54回天然有機化合物討論会・2012年9月・東京

定量NMR法による生薬コロンボ中のcolumbinおよび生薬ゲンチアナ中のgentiopicrosideの定量

○長谷部裕子，田中理恵，永津明人

日本生薬学会第59年会・2012年9月・千葉

生薬アキョウの微量成分

○服部磨依子，田中理恵，永津明人，平雅代，田村真

日本生薬学会第59年会・2012年9月・千葉

qNMRを用いた生薬成分の定量

○永津明人，山崎麻里奈，仁田朱音，長谷部裕子，田中理恵，羽佐田桂子，水上　元，山崎　壮，杉本直樹，西村哲治

第40回生薬分析シンポジウム・2011年12月・大阪

定量NMR法による生薬シャクヤク中のpaeoniflorinの定量

○山崎麻里奈，仁田朱音，羽佐田桂子，田中理恵，永津明人

日本生薬学会第58年会・2011年9月・東京

植物の二次代謝機能を利用した超天然型有用配糖体の創出

○政田さやか，大口幸恵，神永靖久，寺坂和祥，永津明人，水上　元

第50回天然有機化合物討論会・2008年10月・福岡

食品添加物「ブドウ種子抽出物」の品質評価に関する研究

○永津明人，鍵岡明奈，羽佐田桂子

日本生薬学会第55年会・2008年9月・長崎

メシマコブの低分子微量成分の研究

○小島一夫，平山秀樹，荻原幸夫，坂井至通，大野高政，井上誠，水上　元，永津明人

第49回天然有機化合物討論会・2007年9月・札幌

食品添加物「コメヌカ酵素分解物」中のフィチン酸の31P-NMRを用いた定量

○永津明人，羽佐田桂子，水上　元

平成１８年度日本薬学会東海支部例会・2006年12月・名古屋

Identificagtion of the Fused Aromatic Compounds from Phellinus linteus by Detecting the Longer-range Coupling Constants [招待講演]

○Akito Nagatsu

Conference on Natural Products & Biodivesity・2006年11月・DehraDun, India

Lower Molecular Weight Constituents in Phellinus linteus

○Kazuo Kojima, Shizue Itho, Yukio Ogihara, Hajime Mizukami, Akito Nagatsu

IUPAC 25th International Conference on Biodiversity and Natural Products・2006年7月・京都

主な原著論文

Harput Ummuhan Sebnem; Arihan Okan; Iskit Alper B; Nagatsu Akito; Saracoglu Iclal

Antinociceptive, free radical-scavenging, and cytotoxic activities of Acanthus hirsutus Boiss,

Journal of Medicinal Food, (2011) 47, 767-74.

Nagatoshi Mai; Terasaka Kazuyoshi; Nagatsu Akito; Mizukami Hajime

Iridoid-specific glucosyltransferase from Gardenia jasminoides,

Journal of Biological Chemistry, (2011) 286(37), 32866-32874.

Hasada Keiko; Yoshida Takamitsu; Yamazaki Takeshi; Sugimoto Naoki; Nishimura Tetsuji; Nagatsu Akito; Mizukami Hajime

Application of 1H-NMR spectroscopy to validation of berberine alkaloid reagents and to chemical evaluation of Coptidis Rhizoma,

Journal of Natural Medicines, (2011) 65, 262-7.

Yokoi Hiroshi; Mizukami Hajime; Nagatsu Akito; Tanabe Hiroki; Inoue Makoto

Hydroxy monounsaturated fatty acids as agonists for peroxisome proliferator-activated receptors,

Biological & Pharmaceutical Bulletin (2010) 33, 854-61.

Rashmi; Nagatsu, Akito,

New phytochemicals from Xanthocears sorbifolia seeds,

Journal of Medicinal and Aromatic Plant Sciences, (2010) 32, 377-380.

Hasada, Keiko; Yoshida, Takamitsu; Yamazaki, Takeshi; Sugimoto, Naoki; Nishimura, Tetsuji; Nagatsu, Akito; Mizukami, Hajime

Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy,

Journal of Natural Medicines, (2010), 64(2) 161-166.

Yokoi, Hiroshi; Mizukami, Hajime; Nagatsu, Akito; Ohno, Takamasa; Tanabe, Hiroki; Inoue, Makoto

Peroxisome proliferator-activated receptor gamma ligands isolated from adlay seed (Coix lacryma-jobi L. var. ma-yuen stapf.)

Biological & Pharmaceutical Bulletin (2009), 32(4), 735-740.

Kojima, Kazuo; Ogihara, Yukio; Sakai, Yoshimichi; Mizukami, Hajime; Nagatsu, Akito

HPLC profiling of Phellinus linteus

Journal of Natural Medicines (2008), 62(4), 441-446.

Kojima, Kazuo; Ohno, Takamasa; Inoue, Makoto; Mizukami, Hajime; Nagatsu, Akito

Phellifuropyranone A: a new furopyranone compound isolated from fruit bodies of wild Phellinus linteus

Chemical & Pharmaceutical Bulletin (2008), 56(2), 173-175.

Nakagawa, Munehiro; Ohno, Takamasa; Maruyama, Rumi; Okubo, Munenori; Nagatsu, Akito; Inoue, Makoto; Tanabe, Hiroki; Takemura, Genzou; Minatoguchi, Shinya; Fujiwara, Hisayoshi

Sesquiterpene lactone suppresses vascular smooth muscle cell proliferation and migration via inhibition of cell cycle progression

Biological & Pharmaceutical Bulletin (2007), 30(9), 1754-1757.

Rashmi; Dayal, Rameshwar; Nagatsu, Akito

Three oleanolic acid glycosides from the seeds of Achyranthes aspera,

Natural Product Communications (2007), 2(7), 727-730.

Harput, U. Sebnem; Calis, Ihsan; Saracoglu, Iclal; Donmez, Ali A.; Nagatsu, Akito.

Secondary metabolites from Phlomis syriaca and their antioxidant activities.

Turkish Journal of Chemistry (2006), 30(3), 383-390.

Tanabe, H.; Suzuki, H.; Nagatsu, A.; Mizukami, H.; Ogihara, Y.; Inoue, M.

Selective inhibition of vascular smooth muscle cell proliferation by coptisine isolated from Coptis rhizoma, one of the crude drugs composing Kampo medicines Unsei-in.

Phytomedicine (2006), 13(5), 334-342.

Ohno, Takamasa; Nagatsu, Akito; Nakagawa, Munehiro; Inoue, Makoto; Li, Yun-Mo; Minatoguchi, Shinya; Mizukami, Hajime; Fujiwara, Hisayoshi.

New sesquiterpene lactones from water extract of the root of Lindera strychnifolia with cytotoxicity against the human small cell lung cancer cell, SBC-3.

Tetrahedron Letters (2005), 46(50), 8657-8660.

Rana, Virendra S.; Rawat, Mohan S. M.; Pant, Geeta; Nagatsu, Akito.

Chemical constituents and antioxidant activity of Mallotus roxburghianus leaves.

Chemistry & Biodiversity (2005), 2(6), 792-798.

Hirakawa, Kazutaka; Yoshida, Mami; Nagatsu, Akito; Mizukami, Hajime; Rana, Virendra; Rawat, Mohan Singh Maniyari; Oikawa, Shinji; Kawanishi, Shosuke.

Chemopreventive action of xanthone derivatives on photosensitized DNA damage.

Photochemistry and Photobiology (2005), 81(Mar./Apr.), 314-319.

Tanaka, Rie; Sakano, Yuichi; Nagatsu, Akito; Shibuya, Masaaki; Ebizuka, Yutaka; Goda, Yukihiro.

Synthesis of digalactosyl diacylglycerols and their structure-inhibitory activity on human lanosterol synthase.

Bioorganic & Medicinal Chemistry Letters (2005), 15(1), 159-162.

Harput, Sebnem U.; Saracoglu, Iclal; Calis, Ihsan; Donmez, Ali A.; Nagatsu, Akito.

Secondary metabolites from Phlomis kotschyana.

Turkish Journal of Chemistry (2004), 28(6), 767-774.

Nagatsu, Akito; Itoh, Shizue; Tanaka, Rie; Kato, Setsuko; Haruna, Mitsumasa; Kishimoto, Keiichi; Hirayama, Hideki; Goda, Yukihiro; Mizukami, Hajime; Ogihara, Yukio.

Identification of novel substituted fused aromatic compounds, meshimakobnol A and B, from natural Phellinus linteus fruit body.

Tetrahedron Letters (2004), 45(30), 5931-5933.

Nagatsu, Akito; Sugitani, Tomochika; Mori, Yoshiko; Okuyama, Harumi; Sakakibara, Jinsaku; Mizukami, Hajime.

Antioxidants from rape (Brassica campestris vir. Japonica hara) oil cake.

Natural Product Research (2004), 18(3), 231-239.

Harput, U. Sebnem; Nagatsu, Akito; Ogihara, Yukio; Saracoglu, Iclal.

Iridoid glucosides from Veronica pectinata var. glandulosa. Zeitschrift fuer Naturforschung, C:

Journal of Biosciences (2003), 58(7/8), 481-484.

Zhao, Changqi; Nagatsu, Akito; Hatano, Keiichiro; Shirai, Naohiro; Kato, Setsuko; Ogihara, Yukio.

New lignan glycosides from Chinese medicinal plant, Sinopodophillum emodi.

Chemical & Pharmaceutical Bulletin (2003), 51(3), 255-261.

Nagatsu, Akito; Isaka, Kimio; Kojima, Keisuke; Ondognii, Purev; Zevgeegiin, Oyun; Gombosurengyin, Purevsuren; Davgiin, Khishgee; Irfan, Baig; Iqubal, Choudhary Muhammad; Ogihara, Yukio.

New sesquiterpenes from Ferula ferulaeoides (STEUD.) Korovin, VI. Isolation and identification of three new dihydrofuro[2,3-b]chromones.

Chemical & Pharmaceutical Bulletin (2002), 50(5), 675-677.

Matsuno, Michiyo; Nagatsu, Akito; Ogihara, Yukio; Ellis, Brian E.; Mizukami, Hajime.

CYP98A6 from Lithospermum erythrorhizon encodes 4-coumaroyl-4'-hydroxyphenyllactic acid 3-hydroxylase involved in rosmarinic acid biosynthesis.

FEBS Letters (2002), 514(2-3), 219-224.

Isaka, Kimio; Nagatsu, Akito; Ondognii, Purev; Zevgeegiin, Oyun; Gombosurengyin, Purevsuren; Davgiin, Khishgee; Kojima, Keisuke; Ogihara, Yukio.

Sesquiterpenoid derivatives from Ferula ferulioides. V.

Chemical & Pharmaceutical Bulletin (2001), 49(9), 1072-1076.

Tanaka, Rie; Nagatsu, Akito; Mizukami, Hajime; Ogihara, Yukio; Sakakibara, Jinsaku.

Studies on chemical modification of monensin IX. Synthesis of 26-substituted monensins and their Na+ ion transport activity.

Chemical & Pharmaceutical Bulletin (2001), 49(6), 711-715.

Nagatsu, A.; Tanaka, R.; Mizukami, H.; Ogihara, Y.; Sakakibara, J.

Long-range 13C-1H coupling constants (3JC-H) of monensin sodium.

Tetrahedron (2001), 57(16), 3369-3372.

Tanaka, R.; Nagatsu, A.; Mizukami, H.; Ogihara, Y.; Sakakibara, J.

Studies on chemical modification of monensin VIII. Synthesis of 7-O-substituted-25-carboxymonensins and their Ca2+ ion transport activity.

Tetrahedron (2001), 57(15), 3005-3012.

Nagatsu, Akito; Tanaka, Rie; Hashimoto, Masayo; Mizukami, Hajime; Ogihara, Yukio; Sakakibara, Jinsaku.

Studies on chemical modification of monensin. Part 7. Synthesis and Ca2+ ion transport activity of 25-carboxylmonensin.

Tetrahedron Letters (2000), 41(15), 2629-2632.

Zhang, Hui Li; Nagatsu, Akito; Okuyama, Harumi; Mizukami, Hajime; Sakakibara, Jinsaku.

Sesquiterpene glycosides from cotton oil cake.

Phytochemistry (1998), 48(4), 665-668.

Zhang, Hui Li; Nagatsu, Akito; Watanabe, Toshihiro; Sakakibara, Jinsaku; Okuyama, Harumi.

Antioxidative compounds isolated from safflower (Carthamus tinctorius L.) oil cake.

Chemical & Pharmaceutical Bulletin (1997), 45(12), 1910-1914.

Nagatsu, Akito; Nakamura, Yukitaka; Takemoto, Kouya; Nagai, Shin-ichi; Ueda, Taisei; Hatano, Keiichiro; Sakakibara, Jinsaku; Hashimoto, Toshihiro; Asakawa, Yoshinori.

An usual fluoride-mediated oxidation of gitoxin.

Chemical & Pharmaceutical Bulletin (1996), 44(1), 258-60.

Nagatsu, Akito; Kajitani, Hitoshi; Sakakibara, Jinsaku.

Muscoride A: a new oxazole peptide alkaloid from freshwater cyanobacterium Nostoc muscorum.

Tetrahedron Letters (1995), 36(23), 4097-100.

Uemura, Daisuke; Chou, Tong; Haino, Takeharu; Nagatsu, Akito; Fukuzawa, Seketsu; Zheng, Shu-zhen; Chen, Hai-sheng.

Pinnatoxin A: a toxic amphoteric macrocycle from the Okinawan bivalve Pinna muricata.

Journal of the American Chemical Society (1995), 117(3), 1155-6.

Nagatsu, Akito; Watanabe, Miyako; Ikemoto, Kouji; Hashimoto, Masayo; Murakami, Nobutoshi; Sakakibara, Jinsaku; Tokuda, Harukuni; Nishino, Hoyoku; Iwashima, Akio; Yazawa, Kazunaga.

Synthesis and structure-antitumor-promoting activity relationship of monogalactosyl diacylglycerols.

Bioorganic & Medicinal Chemistry Letters (1994), 4(13), 1619-22.

last update 8/23, 2012